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Achieve high efficiency of probe-based qPCR under fast or standard cycling conditions. Minimize troubleshooting─PrimeTime qPCR Probes are quality controlled via electrospray ionization mass spectrometry (ESI-MS)* and HPLC-purified.
- qPCR Primers
Figure 1. PrimeTime qPCR Primer Assays yield the same high...
- PrimeTime Gene Expression Master Mix
Figure 2. Reliable results from single-plex (black) and...
- PrimeTime qPCR Probe Assays
PrimeTime qPCR Probe Assays consist of a primer pair and...
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Shinjuku I-Land Tower 25F 6-5-1 Nishi-Shinjuku Shinjuku-ku...
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Find Archer now at IDT! All Archer information is now...
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We now offer Affinity Plus qPCR Probes — a new locked...
- Sun Fluorophore
Figure 3. PrimeTime SUN-labeled probes deliver comparable...
- Phosphorothioate Bonds
Phosphorothioate bonds can be introduced between the last...
- qPCR Primers
Order PrimeTime™ qPCR Probes, 5' nuclease probes in a range of formats and dye-quencher combinations.
PrimeTime qPCR Probe Assays consist of a primer pair and fluorescently labeled 5′ nuclease probe. Obtain predesigned sequences for human, mouse, or rat for easy selection based on multiple criteria such as exon location and number of transcripts. Create custom assays for any sequence from any species using the PrimerQuest™ Tool. qPCR solutions.
7 de abr. de 2015 · PrimeTime™ qPCR Assays. Predesigned probes and primers for probe-based or intercalating dye-based qPCR available in multiple scales and formats. Choose from predesigned sequences for human, mouse, or rat, or create a custom assay for any sequence from any species.
PrimeTime qPCR 5' Nuclease Assays are offered in three different sizes to meet any qPCR experimental need. In addition, for the Standard and XL sizes, selection of dye–quencher combination and primer-to-probe ratio can be specified to meet unique experimental demands.
Schematic of a PrimeTime® qPCR 5’ Nuclease Assay using a double- quenched probe that includes a dye, a ZEN ™ or TAO internal quencher, and a 3’ quencher.
Schematic overview of a PrimeTime® qPCR 5’ Nuclease Assay using a double-quenched probe that includes a 5’ dye, a ZEN™ or TAO™ internal quencher, and a second 3’ quencher. With nearly 4 times lower background fluorescence (Figure 3A) and approximately 30% increased signal (Figure 3B), ZEN Double-Quenched Probes simply perform better.
