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relacionado con: quick change mutagenesis kitEfficient single site mutagenesis - QuikChange II delivers 80%+ mutation efficiency. Complete kit provides all reagents necessary to support mutagenesis. Learn more.
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The QuikChange II system is the second generation of Agilent’s QuikChange method. It provides improved fidelity over the original kit while maintaining greater than 80% mutation efficiency for single site mutagenesis. The kit includes PfuUltra High-Fidelity DNA Polymerase to minimize unwanted errors.
The QuikChange II site-directed mutagenesis kit is used to make point mutations, replace amino acids, and delete or insert single or multiple adjacent amino acids.
QuikChange Site-Directed Mutagenesis Kit. This kit uses PfuTurbo DNA polymerase. It replicates plasmid strands with high fidelityll without displacing mutant oligonucleotide primers. The process uses a supercoiled dsDNA vector with insert and two synthetic oligo primers containing the mutation.
The QuikChange Multi Site-Directed Mutagenesis Kit offers a rapid and reliable method for site-directed mutagenesis of plasmid DNA at up to five different sites simultaneously.
The QuikChange II-E site-directed mutagenesis kit is used to make point mutations, replace amino acids, and delete or insert single or multiple adjacent amino acids.
The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA.
aThe QuikChange Site-Directed Mutagenesis Kits contain enough reagents for 5 control reactions. bSee Preparation of Media and Reagents. cThaw the dNTP mix once, prepare single-use aliquots, and store the aliquots at –20°C. Do not subject the dNTP mix to multiple freeze-thaw cycles.
