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The QuikChange II system is the second generation of Agilent’s QuikChange method. It provides improved fidelity over the original kit while maintaining greater than 80% mutation efficiency for single site mutagenesis. The kit includes PfuUltra High-Fidelity DNA Polymerase to minimize unwanted errors.
- QuikChange Primer Design
The QuikChange® Primer Design Program supports mutagenic...
- Nucleic Acid Calculators
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- QuikChange Primer Design
The QuikChange II site-directed mutagenesis method is performed using Pfu Ultra high-fidelity (HF) DNA polymerase for mutagenic primer-directed replication of both plasmid strands with the highest fidelity.
The QuikChange II site-directed mutagenesis kit is used to make point mutations, replace amino acids, and delete or insert single or multiple adjacent amino acids.
Our QuikChange Site-Directed Mutagenesis Kit allows site-specific mutation in virtually any double-stranded plasmid, thus eliminating the need for subcloning into M13-based bacteriophage vectors and for ssDNA
The highest fidelity site-directed mutagenesis kit allows you in 3 easy steps to introduce a variety of mutations in any vector in a single day. Includes all necessary components required to perform 10 reactions.
This rapid four-step procedure generates mutants with greater than 80% efficiency. The protocol is simple and uses either miniprep plasmid DNA or cesium-chloride-purified DNA. For long (~8 kb) or difficult targets, Stratagene offers the QuikChange® XL site directed mutagenesis kit (Catalog #200516).
The QuikChange Multi Site-Directed Mutagenesis Kit offers a rapid and reliable method for site-directed mutagenesis of plasmid DNA at up to five different sites simultaneously.
