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  1. The PrimeTime Eco qPCR Probe is the ideal scale when you need to perform ~500 reactions for gene expression analysis or genotyping. The combination of medium scale and low cost is ideal for initial screening of large sample sets.

    • View FAQ

      View FAQ - qPCR Probes PrimeTime | IDT - Integrated DNA...

    • Order Now

      We now offer Affinity Plus qPCR Probes — a new locked...

    • Sun Fluorophore

      Figure 3. PrimeTime SUN-labeled probes deliver comparable...

    • Phosphorothioate Bonds

      Phosphorothioate Bonds - qPCR Probes PrimeTime | IDT -...

  2. PrimeTime qPCR Probe Assays consist of a primer pair and fluorescently labeled 5nuclease probe. Obtain predesigned sequences for human, mouse, or rat for easy selection based on multiple criteria such as exon location and number of transcripts.

  3. PrimeTime™ qPCR Probes. PrimeTime qPCR probes are customized to your specifications and include several probe types, including PrimeTime double-quenched probes, Affinity Plus LNA Probes, and molecular beacon probes.

  4. We now offer Affinity Plus qPCR Probes — a new locked nucleic acid modified qPCR probe. These affordable qPCR probes can increase Tm to provide greater sensitivity and mismatch discrimination. Find out more here.

  5. Premixed primer pairs for analyzing gene expression using intercalating dyes. PrimeTime qPCR Primer Assays provide a primer pair designed for real-time PCR using intercalating dyes, such as SYBR ® Green (Thermo Fisher Scientific) or EvaGreen ® (Biotium) dyes.

  6. Predesigned qPCR Probe sequences for human, mouse, and rat. Design custom sequences using IDTs PrimerQuest Design Tool or use your own sequence.

  7. PrimeTime qPCR Probes provide reliable sensitivity even in demanding applications such as multiplexing and digital PCR. PrimeTime qPCR Probes are available in a wide variety of dye-quencher combinations (Figure 1) that are compatible with common qPCR instruments. Achieve consistent results