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filter paper bridges. Extra medium was added, or embryos were transferred to larger containers when required. Initially embryos were placed intact into the medium, but to obtain good contact between axis and medium, one or both cotyledons were removed from the larger embryos after several weeks culture.
At the present work, the utility of zygotic embryo culture as a method for breeding purpose in avocado was evaluated. In vitro germination and rooting of zygotic embryos, sprout multiplication and plantlet adaptation of Cuban avocado varieties were studied.
1 de oct. de 1990 · After 2 weeks, the somatic embryos were transferred to paper bridges with the same medium (liquid) for 45 days and were allowed to mature into young plantlets. The plantlets, which were of various sizes, were removed from the vials and sep- arated into two size categories: 2,4 and 5-7 cm in length.
1 de ene. de 2020 · Embryo culture conditions have been standardized for culturing the mature and immature embryos in chickpea, and liquid media with filter paper bridge setup have been identified to be congenial to support the growth of the immature embryo.
A new filter paper bridge technique was compared with the existing agarmedium for callus initiation, multiplication, and plant regeneration of wheat and the yield of both total and embryogenic callus was doubled and significantly higher number of regenerants was obtained on filter paper bridges compared to agar medium. Expand.
9 de feb. de 2019 · In the Agrobacterium tumefaciens-mediated transformations of maize immature embryos (IEs), the common co-culturing media used are MS or N6-based (MC). Here, we used a novel co-culturing method in which maize ‘Qi319’ IEs inoculated with Agrobacterium-harboring target vector were placed on dry filter paper (DC) in a petri dish.
31 de dic. de 2020 · Developed embryos and/or embryogenic calli were transferred to filter paper bridges in glass tubes (20 mm × 200 mm) with a liquid MSm medium of the same content and cultured at the same conditions for 7–8 weeks.
