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23 de feb. de 2021 · Our protocol is composed of five main steps, including protoplast isolation, protoplast cell proliferation, microcallus formation, de novo shoot regeneration, and de novo root regeneration. Each step was optimized by examining multiple culture conditions.
2 de sept. de 2021 · The use of transient transformation and regeneration of plant protoplasts is one such strategy that carries unique advantages and challenges. Here, we will discuss the use of protoplast regeneration in the application of new plant breeding technologies and review pertinent literature on successful protoplast regeneration.
7 de jul. de 2021 · Polyethylene glycol (PEG)-mediated protoplast transfection is an alternative for delivery of CRISPR vectors or ribonucleoprotein complexes (RNPs) into plant cells, which can produce transgene-free mutation lines through transient gene expression.
27 de ago. de 2023 · In this study, factors affecting protoplast regeneration such as the composition of the enzyme solution (concentration and choice of cell-wall digesting enzymes) for successful protoplast isolation and different culture protocols were tested.
5 de mar. de 2024 · Here we highlight the current research advances in protoplast transformation and discuss how to optimize the procedures of protoplast isolation, transfection, and regeneration for efficient and reproducible CRISPR/Cas-based genome editing in the genus Solanum.
16 de nov. de 2021 · In this study, we have systemically investigated some important factors affecting protoplast culture and regeneration, and have established a highly efficient and relatively simple protocol for protoplast regeneration and transfection for the species.
3 de sept. de 2021 · Here, we will discuss the use of protoplast regeneration in the application of new plant breeding technologies and review pertinent literature on successful protoplast regeneration. Keywords: crop improvement; gene editing; protoplast; regeneration; tissue culture.
