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Gels can either use a single concentration of acrylamide where they are optimised for a narrow range of molecular weights (see section 1) or be gradients of acrylamide concentration where they are optimised for a much wider range of weights (see section 2).
Polyacrylamide gels are characterized by two parameters: total monomer concentration (%T, in g/100 ml) and weight percentage of crosslinker (%C). By varying these two parameters, the pore size of the gel can be optimized to yield the best separation and resolution for the proteins of interest.
The pore size of a gel is determined by two factors: the total amount of acrylamide present (designated as %T) and the amount of cross-linker (%C). As the total amount of acrylamide increases, the pore size decreases.
Since your protein is relatively big, approx. 10% or lower fixed percentage acrylamide gels would do the job. In the case of other targets along with 88kda protein as Adam B Shapiro...
Agarose was usedcyanol FF to prepare the gels. • Only freshly prepared electrophoresis buffers should be used. The buffers were prepared from 50XTAE Buffer and 10X TBE Buffer. • Choose electrophoresis conditions according to the recommendations below: Size of the DNA Voltage Buffer <1 kb 5-10 V/cm TBE 1-5 kb 4-10 V/cm TAE or TBE > 5 kb 1-3 ...
To obtain the best result based on your application (PCR fragment separation, DNA retardation, etc.), it is important to choose the correct gel percentage, buffer system, gel format, and thickness. Figure 1 shows the migration pattern of nucleic acids run on Novex gels.
Calculate Polyacrylamide gel recipes for SDS-PAGE. Just enter the number of gels (18x16mm) and the percent polyacrylamide needed
