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Hace 5 días · While the quantity of DNA was not in the detection range of current DNA measurement technologies for 20 and 50 µl samples, those of 100 and 200 µl samples had a mean DNA concentration range of 0 ...
Hace 4 días · DNA amplification was performed in the total reaction volume. The amplified product was electrophoresed and visualized after cycles of denaturation, annealing, and extension. A positive test was indicated by the presence of a 240 bp fragment. ... For lung tissue samples, tNGS demonstrated statistically significant differences (p < 0.05) ...
Hace 4 días · This guide will provide tips and tricks to prepare a high-quality single-cell suspension that is broadly applicable to most cell and tissue types. The first part of this guide will cover the initial stages of sample selection and general best practice in experimental planning and organization. The second part will cover practical cell handling ...
Hace 3 días · Cell free DNA (cfDNA)-based assays hold great potential in detecting early cancer signals yet determining the tissue-of-origin (TOO) for cancer signals remains a challenging task. Here, we investigated the contribution of a methylation atlas to TOO detection in low depth cfDNA samples.
Hace 3 días · Southern blot analysis is a laboratory method used to study DNA. Specifically, purified DNA from a biological sample (such as blood or tissue) is digested with a restriction enzyme(s), and the resulting DNA fragments are separated by using an electric current to move them through a sieve-like gel or matrix, which allows smaller fragments move ...
Hace 1 día · Pediatric central nervous system tumors remain challenging to diagnose. Imaging approaches do not provide sufficient detail to discriminate between different tumor types, while the histopathological examination of tumor tissue shows high inter-observer variability. Recent studies have demonstrated the accurate classification of central nervous system tumors based on the DNA methylation profile ...
Hace 3 días · In situ hybridization is a laboratory technique used to localize a sequence of DNA or RNA in a biological sample. In this technique, a biological sample consisting of tissue sections, cells or chromosomes from an individual is affixed to a glass slide and then exposed to a “probe”—a small piece of single-stranded DNA tagged with a ...
