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  1. www.ncbi.nlm.nih.gov › tools › primer-blastPrimer designing tool - National Center for Biotechnology...

    Primer-BLAST allows you to design primers for PCR based on a template sequence, using Primer3 and BLAST algorithms. You can customize the search parameters, such as primer length, melting temperature, exon/intron selection, and specificity checking.

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  3. www.ncbi.nlm.nih.gov › guide › howtoDesign PCR primers and check them for specificity

    Primer BLAST is a tool that helps you design and evaluate PCR primers based on a target sequence or a template sequence. You can enter one or both primer sequences, select the organism and database, and get primer pairs and specificity information.

  4. www.ncbi.nlm.nih.gov › tools › primer-blastPrimer-Blast results - National Center for Biotechnology...

    Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target.

  5. www.ncbi.nlm.nih.gov › pmc › articlesPrimer-BLAST: A tool to design target-specific primers for...

    18 de jun. de 2012 · We present a new software tool called Primer-BLAST to alleviate the difficulty in designing target-specific primers. This tool combines BLAST with a global alignment algorithm to ensure a full primer-target alignment and is sensitive enough to detect targets that have a significant number of mismatches to primers.

  6. www.ncbi.nlm.nih.gov › tools › primer-blastNational Center for Biotechnology Information

    Primer-BLAST helps users make primers that are specific to intended PCR target by using Primer3 and BLAST algorithms. It screens primers against user-selected database to avoid non-specific amplifications and provides citation and details.

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  8. www.ncbi.nlm.nih.gov › tools › primer-blastNational Center for Biotechnology Information

    Allow primers to amplify mRNA splice variants. This will make it much easier to find gene-specific primers if you do not need to distinguish between splice variants. Specify an organism for database search if you are only amplifying DNA from a specific organism.

  9. www.nlm.nih.gov › ncbi › workshopsThe Primer-BLAST interface - National Library of Medicine

    16 de oct. de 2022 · The Primer-BLAST interface. The Primer-BLAST input form has four main sections: Sequence input (PCR Template and Primer Parameters) Exon / Intron selection. Primer Pair Specificity Checking Parameters (BLAST database selection and options) Advanced Parameters.