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23 de feb. de 2021 · This study developed a reproducible and efficient method for protoplast regeneration in Arabidopsis, using a modified alginate-embedding technique. The method involves four steps: protoplast isolation, cell division, callus formation, and de novo shoot and root regeneration.
2 de sept. de 2021 · The use of transient transformation and regeneration of plant protoplasts is one such strategy that carries unique advantages and challenges. Here, we will discuss the use of protoplast regeneration in the application of new plant breeding technologies and review pertinent literature on successful protoplast regeneration.
7 de jul. de 2021 · Polyethylene glycol (PEG)-mediated protoplast transfection is an alternative for delivery of CRISPR vectors or ribonucleoprotein complexes (RNPs) into plant cells, which can produce transgene-free mutation lines through transient gene expression.
27 de ago. de 2023 · The article reports on the optimization of protoplast isolation protocol and the regeneration of different cabbage cultivars. It discusses the factors affecting protoplast yield, viability, and regeneration efficiency, and the applications of protoplast technology in plant biotechnology.
5 de mar. de 2024 · Here we highlight the current research advances in protoplast transformation and discuss how to optimize the procedures of protoplast isolation, transfection, and regeneration for efficient and reproducible CRISPR/Cas-based genome editing in the genus Solanum.
16 de nov. de 2021 · In this study, we have systemically investigated some important factors affecting protoplast culture and regeneration, and have established a highly efficient and relatively simple protocol for protoplast regeneration and transfection for the species.
3 de sept. de 2021 · Here, we will discuss the use of protoplast regeneration in the application of new plant breeding technologies and review pertinent literature on successful protoplast regeneration. Keywords: crop improvement; gene editing; protoplast; regeneration; tissue culture.
