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Learn how to perform Golden Gate assembly, a one-pot, one-step cloning method using Type IIS restriction enzymes. Find out the components, steps, and kits for Golden Gate assembly and simulate your experiment with Snapgene.
Golden Gate Cloning or Golden Gate assembly is a molecular cloning method that allows a researcher to simultaneously and directionally assemble multiple DNA fragments into a single piece using Type IIS restriction enzymes and T4 DNA ligase.
Learn how to use NEB's kits and tools for efficient and seamless assembly of DNA fragments using Type IIS restriction enzymes. Golden Gate Assembly is a versatile method for cloning, library creation, and gene editing applications.
2 de nov. de 2022 · Golden Gate cloning is used for assembly of multiple DNA fragments in a defined linear order in a recipient vector using a one-pot assembly procedure. Golden Gate cloning is based on the use of a type IIS restriction enzyme for digestion of the DNA fragments and vector.
Learn how to use Golden Gate Assembly, a method for joining multiple DNA fragments with Type IIS endonucleases, in this video tutorial. See how to design primers, perform PCR, and transform the assembled products.
For your convenience, NEB now ofers two kits for Golden Gate Assembly featuring BsaI-HFv2 or BsmBI-v2. Both kits incorporate digestion followed by ligation with T4 DNA Ligase into a single reaction, and can be used to assemble 2-50+ fragments in a single step.
Snapgene offers a video series to teach you how to perform Golden Gate assembly, a fast and versatile cloning technique. Learn the basics, the pros and cons, and the tips for Golden Gate assembly with Type IIS enzymes.
